Fluorescence microscopy techniques are powerful tools for probing very small signals and revealing three-dimensional (3D) structural and functional properties of biological samples with high ...

Recent advances in electron microscopy and diffraction have increasingly focused on capturing dynamical processes at unprecedented temporal resolutions. Ultrafast electron microscopy and diffraction ...

Raman microscopy combines optical microscopy with the ability to determine the chemical makeup of surfaces. Laser light is delivered to the sample surface via the same objective as the optical light ...

A decade ago, the Nobel Prize in Chemistry was awarded to a trio of researchers for the development of super-resolved fluorescence microscopy. The announcement at the time stated that the researchers’ ...

While confocal microscopy uses a pinhole to reject out-of-focus light to generate the optical section, a multi-photon (or 2-photon) microscope uses a pulsed infrared laser to stimulate fluorescence ...

Stimulated emission depletion (STED) microscopy has shown that diffraction-unlimited spatial resolution is viable with conventional lenses and visible light. Stefan W Hell, Lars Kastrup and Katrin I ...

A research team has created a fluorescent marker molecule that does not degrade under a STED microscope: the photobleaching problem has been solved. Images of living cells can now be captured over ...

Nanoscopy is a field of microscopy that focuses on imaging and studying structures and processes at the nanoscale, typically below the diffraction limit of light. It encompasses various techniques ...

It’s relatively easy to understand how optical microscopes work at low magnifications: one lens magnifies an image, the next magnifies the already-magnified image, and so on until it reaches the eye ...

Fluorescence light microscopy techniques offer several advantages when imaging biological samples, including high image contrast, good labeling specificity, multicolor, and three dimensional (3D) ...

Visualizing tissues and neurons inside an animal's skull has long been a challenge for biophotonics techniques, thanks to the skull's thickness and the complex structures inside. A team at the ...